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WebApr 29, 2024 · Culture medium of SMCs and 3T3s was collected and analysed using Sircol soluble collagen assay as described by the manufacturer (Biocolor, S1000). In comparable subsequent experiments, transcription growth factor (TGF)-β1 was added to the conditioned medium or proteins were heat-inactivated (30 min, 85°C) prior to addition to 3T3 fibroblasts. Webto soluble denatured extraction, (S1000 Assay) and place into a screw capped collagen round bottom 2 ml digestion tube (as supplied in the S2000 Assay Kit). ... unbound … hiller heating and cooling knoxville
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