Web一、 脉冲追踪. 这是一种追踪蛋白质并估计其稳定性的传统方法,除了涉及到处理放射性,这种方法在测量蛋白质半衰期时比其他药理方法具有明显的优势,在实验室中仍然经常使用。. 它不仅能测量蛋白质半衰期,而且如果 … WebCycloheximide 是真核细胞中的一种蛋白质合成抑制剂。. 虽然其确切的作用机制仍完全难以捉摸,但研究表明它能通过结合 60S 核糖体单位的 E 位点并干扰去乙酰化的 tRNA 来抑制翻译延伸 (1-3)。. 尽管并非所有细胞类型 …
Cycloheximide Cell Signaling Technology
WebJun 13, 2003 · RL34 cells were pretreated with MG132 for 2 h before the proteasome inhibitor was removed and replaced with media containing CHX and either Sul or vehicle. When cotreated with CHX and vehicle, a notable reduction in the expression of rNrf2 was observed after a chase period of 60 min (Fig. 4C), indicative of proteasomal-mediated … WebNov 4, 2015 · Cycloheximide (Naramycin A) 是一种抗真菌 (antifungal) 抗生素,是真核生物蛋白质合成 (protein synthesis) 的抑制剂,抑制体内蛋白质合成和 RNA 合成的 IC50 值 … phlebotomy pillow
Cycloheximide Chase Analysis of Protein Degradation in ... - PubMed
WebJan 12, 2024 · Mix carefully and allow to stand at room temperature for 10 min to precipitate the RNA. Centrifuge the mixture at 12,000 x g for 10 min at 4 °C. Remove the supernatant and wash the RNA pellet by adding 1 … WebAfter 24 h, 100µg/ml cycloheximide (CHX) was added to the culture medium and cell lysates were harvested at indicated times after the CHX addition. Cell lysates were then subjected to immunoblot ... WebFeb 10, 2024 · 脉冲一示踪标记法 (pulse- chase labelingasy) 是用核素或其他标记物标记细胞或生物个体的特定代谢物,通过自显影等方法追踪分析某一代谢过程的技术。. 例如,用放射性核素标记氨基酸,可检测某种特定蛋白质在细胞内的变化过程。. 原理. 脉冲追踪标记法 … tst leye - bub city