WebNov 20, 2024 · In water, its maximum absorption/emission is 494/518 nm, corresponding to the color blue and green. The blue color spectrum is 450-495 nm, and the green spectrum is 495-570 nm. Fluorescein isothiocyanate (FITC) is the derivative of fluorescein with an isothiocyanate reactive group, rendering it reactive towards amine and sulfhydryl groups ... WebJan 10, 2024 · Alexa Fluor® dyes are a big group of negatively charged and hydrophilic fluorescent dyes, frequently used in fluorescence microscopy. All the Alexa Fluor® dyes are sulfonated forms of different basic fluorescent substances like fluorescein, coumarin, cyanine or rhodamine (e.g. Alexa Fluor®546, Alexa Fluor®633).The respective laser …
A Guide to Fluorochromes - Abcam
WebAbsorption (top) and emission (bottom) spectra of Vio Bright FITC compared to FITC, Alexa Fluor 488, and BD Horizon Brilliant Blue 515 (BB515). ... showing maximum absorption around 652 nm. Emission … WebTo only list those, select '2-Photon Cross-Sections' from the drop down menu. Unfortunately, also all one-photon spectra for the same dyes are shown in the list, so you may have to search a bit. The 2P-spectrum is sometimes included with the one-photon excitation (e.g. Cy5, Cy5.5). Usually, however, the two photon spectrum is indicated with -2P ... eastlea tabernacle
UV – vis absorption spectra of pure FITC, plain silica and …
WebFor absorbency spectrum, the solution has all the wavelengths shining on the solution, and you monitor the wavelengths of interest. The absorbency shows which wavelengths are absorbed by the solution. The excitation … WebThe absorption spectrum of a typical fluorochrome is illustrated in Figure 1(a) where the relative intensity of absorption is plotted against the measured wavelength. A monochromator (a device that allows … WebThis filter set is designed for optimal detection of the traditional fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) probes in combination, as well as other fluorophores with similar … eastlea school newham