WebFeb 4, 2024 · PhosTag electrophoresis and immunoblotting show the phosphorylation status of cGAS and pH3S10, respectively. (G) cGAS-deficient HeLa cells were reconstituted with WT cGAS or the cGAS 8A mutant with all eight N-terminal phosphorylation sites replaced with alanine. These cells were arrested with thymidine and nocodazole before … Web1. SDS-PAGE胶中的Phos-tag™ 捕获磷酸化蛋白。. 2. 电泳中的磷酸化蛋白的泳动速度变慢。. 3. 根据磷酸化水平出现在上移后的位置，分离出磷酸化蛋白的条带。. Phos-tag™ 是日本广 …
Phos-tag gel: does anybody have experience with it?
WebJul 2, 2012 · Dhiya Dalila Zawawi. I had run a phos-tag electrophoresis with resolving gel contain 6% of acrylamide, 50 uM of phos-tag, 100 µM ZnCl2, TEMED, 0.35M of Bis-Tris base and APS. I ran the gel at ... http://www.ebiotrade.com/custom/Boppard/191105/index.html ios icon packs free reddit
一种全新的磷酸化蛋白检测方法——Phosbind_哔哩哔哩_bilibili
WebPhos-tag™ Fluorescent Gel Stain Reagent is a reagent for fluorescent staining of phosphorylated proteins in a gel and one that can work at physiological pH. This product specifically stains phosphorylated proteins when applied to a gel after SDS-PAGE. WebAbstract. Phos-tag gels are recent tools to dissect protein phosphorylation that operate by inducing a shift in the electrophoretic mobility of phosphorylated proteins compared to … WebUsing 2 different PhosTag batches keeping all the rest the same (i.e., acrylamide sol., APS/TEMED/ buffers, etc) yielded two gels that gave markedly different behaviour. The first gel (with the ... ios ics importieren