Pslf172

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August undefined, 2024

WebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. WebMolecular Biology of the Cell Vol. 13, 1132–1143, April 2002 The Schizosaccharomyces pombe Aurora–related Kinase Ark1 Interacts with the Inner Centromere Protein Pic1 and Mediates Chromosome Segregation

The fission yeast homologue of Orc4p binds to replication origin …

WebpSLF172 87609 ™ Clone type Vector Applications Molecular biology Product format Freeze-dried See Additional Product Information Buy Now Price: $590.00 EA Discounts may be … Webresultant fragments were cloned into the PstI and BglII sites of pSLF172 (63), which was designed to add a 3xHA tag at the C terminus of the respective protein. Since the plasmids obtained (pSLF172-PKC1, pSLF172-PKC1T1125A, and pSLF172-PKC1S1143A) lacked the terminator region hot air balloon pics

The Schizosaccharomyces pombe Aurora–related Kinase Ark1 …

WebJan 15, 2002 · To construct the pSLF172-81X-sec31 expression plasmid, the sec31 ORF was first PCR amplified and cloned into the ura4 based plasmid pSLF173 (Forsburg and Sherman, 1997) to create pSLF173-sec31. A LEU2 marked, low-strength nmt1 -controlled expression plasmid was constructed by cleaving pSLF172 ( Forsburg and Sherman, 1997 … WebDec 22, 2000 · A NotI linkered genomic MPK4 fragment including the 1150 bp promoter was amplified from La-0 genomic DNA and cloned into that site of pSLF172 (Forsburg and Sherman 1997) to produce a C terminally triple HA-tagged MPK4. The activation loop mutant (T201A/Y203F) was made using the QuickChange kit (Stratagene). hot air balloon piggy bank

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Addgene: Vector Database - pSLF172,272,372

WebJun 3, 1997 · As shown in Fig. 2 A, an anti-Cdc19p antibody recognizes a low amount of endogenous Cdc19p protein in the control lysate, and substantially increased amounts in … WebThe hsk1 + open reading frame was amplified using PCR of S. pombe cDNA and cloned into pSLF172 ( 30 ) to give pSLF172-hsk1. The first 575 base pairs of the hsk1 + open reading frame, the nmt1 + promoter, and the ars1 sequences were removed from pSLF172-hsk1 by restriction enzyme cleavage and re-ligation. psychotherapeut frohburgWebOct 28, 2005 · Next, GFP was isolated as an NcoI–NotI fragment from pcpy-GFP and ligated together with XhoI/NcoI digested PCR product and XhoI/NotI digested pSLF172, … psychotherapeut gehalt bayern

"WebJun 10, 1997 · The source of Cig2 kinase, GBY228, is a strain carrying a plasmid expressing the cig2 + ORF fused at its C terminus to a triple hemaglutinin (HA) tag from the nmt1 … " - Pslf172

Pslf172

WebThe vector was constructed by cutting pSLF172 with SacI and XhoI, isolating the fragment containing the HA polylinker, and ligating into XhoI-SacI digested REP42X. Mycoplasma … WebJun 1, 1997 · The source of Cig2 kinase, GBY228, is a strain carrying a plasmid expressing the cig2+ ORF fused at its C terminus to a triple hemaglutinin (HA) tag from the nmt1 promoter in the vector pSLF172 ( 14 ). The strain expressing HA-tagged Cdc13, GBY248, was constructed in a similar manner.

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WebPlasmid pREP3‐adh: The nmt1‐MCS‐3xHA‐term cassette from pSLF172 (Forsburg and Sherman, 1997) was cloned as a PstI/SacI fragment into pREP3X (gift of S.L. Forsburg), giving rise to plasmid pREP3X‐tag. Subsequently a XhoI/NotI fragment from pREP3X‐tag was substituted with a hybridized tw75/tw76 primer adapter giving pREP3‐SX. WebTo construct pSLF172 (L)-pka1 plasmid, the pka1 gene from the plasmid pSLF372(L)-pka1 was digested with the restriction enzymes BglII and NotI. The digested fragment was …

WebClick here to find the location of this part on the equipment Part Number: 7017172, Description: Assembly, Counterbalance Valve, JLG Parts. Using genuine JLG parts is the … WebELLULAR DNA replication requires precise regu- lation to ensure that the genome is replicated once completely in each cell cycle, and that the process of replication is coordinated with proper cell cycle entry and subsequent nuclear division.

WebPurification of Hsk1, a Minichromosome Maintenance Protein Kinase from Fission Yeast* (Received for publication, February 6, 1998, and in revised form, June 16, 1998) WebOct 13, 2024 · Thecdc19-M4 mutant was constructed and cloned into the HA-tagging nmt1 + expression vector pSLF172 (Forsburg and Sherman, 1997) as described previously (Forsburg et al., 1997). Plasmid pMF56, expressing N-terminally HA-tagged Cdc18p under control of the nmt1 + promoter was a kind gift of Marco Muzi-Falconi and Tom Kelly (Muzi …

WebJun 10, 2005 · The synthesis of O-mannosyl glycans is initiated in the endoplasmic reticulum (ER) by the transfer of a mannosyl residue from dolichyl phosphate-activated mannose (Dol-P-Man) to specific serine/threonine residues of proteins entering the secretory pathway ( Strahl-Bolsinger et al ., 1999; Willer et al ., 2003 ).

WebPoint mutations in pSLF172-hsk1 were generated using QuickChange PCR (Stratagene). Transformants were isolated in the presence of 5 μg/ml thiamine, and expression was … psychotherapeut gehalt hamburgWebJan 15, 2011 · To construct strains with tagged episomal versions of Cdc18 and Swi6, the cdc18 + open reading frame was amplified from S. pombe cDNA using PCR and cloned as a Xho1/Sma1 fragment into the pSLF172 vector 58 downstream of the nmt1 promoter, generating a Cdc18 tagged with the triple HA epitope sequence at the C-terminus. hot air balloon pilotWebMar 17, 2014 · 2003). 3HA tag was fused in frame to the C-terminus of fission yeast Rec8 protein using pSLF172 (Forsburg & Sherman 1997). All strains used in this study are listed in Table S3 (Supporting Information). Synchronous meiotic cultures of budding and fission yeast and monitoring meiotic progression were conducted as described previously (Ohta … hot air balloon pilot training canada