Webb11 apr. 2024 · IntroductionProbiotics play critical roles in relieving inflammatory bowel disease (IBD). However, the underlying mechanism of Bacteroides fragilis strain ZY-312 (B. fragilis) for colonic mucosa regeneration in IBD remains unclear.MethodsThe weight loss, disease activity index (DAI), colon length, and histopathology-associated index (HAI) … Webb27 jan. 2024 · The small intestine is composed of villi that project into the lumen of the gut and crypts that ... < 0.05. B. Left: Dividing (EdU positive cells) in the monolayer of epithelial cells stained 14 days after seeding the organoids over the collagen scaffold containing fibroblasts with fluid shear stress. Top view, maximal projection of ...
Developing an advanced gut on chip model enabling the study of ...
Webb30 okt. 2024 · As shown in Figure 6A,B, organoids derived from both Shp-2 IEC-E76K small intestine and colon developed much more rapidly in comparison with organoids derived from control mice. Furthermore, activated Shp-2 promoted de novo crypt formation in enteroids, as demonstrated by increased numbers of protrusions compared with … WebbStaining was visualized with Alexa-conjugated secondary antibody (1:500, 1 h at RT) after which the tissue slides were mounted with ProLong™ Gold antifade reagent with DAPI (Invitrogen). Images were obtained on a Leica CTR 6000 and processed with ImageJ (version 1.52a, National Institutes of Health). small custard bowls
Protocol Guide: Immunofluorescent Staining of Whole …
Webb23 feb. 2024 · Effects of steroid hormone and PGE2 treatment on human endometrial epithelial organoids. (A) Organoids were seeded in Matrigel and grown for 4 days in expansion media (ExM), followed by treatment with vehicle as a control or estrogen (E2) for 2 days.Next, the ExM was replaced with base media, and organoids were treated for 6 … WebbHistology staining of jejunal and duodenal sections of small intestines The jejunal and duodenal sections of small intestines of indomethacin-treated mice were surgically removed and fixed in zinc formalin for 2 hours followed … Webb8 mars 2024 · In human small intestinal organoids, two distinct morphologies have been reported, cystic and multilobular 27, 28. Multilobular organoids have one or multiple … sona mens watches