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Rational Protein Engineering of Thermo-Stable PETase from
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Webb10 nov. 2024 · Here, Is PETase, derived from the bacterium Ideonella sakaiensis, is considered to be the most active enzyme for PET degradation under mild conditions, and although several studies have demonstrated improvements to both the stability and activity of this enzyme, stability at even moderate temperatures is still an issue. Webb17 apr. 2024 · PETase catalyzes the depolymerization of PET to bis (2-hydroxyethyl)-TPA (BHET), MHET, and TPA. MHETase converts MHET to TPA and EG. Open in viewer Beyond PET, humankind uses a wide range of polyesters, broadly classified by aliphatic and aromatic content. PET, for example, is a semiaromatic polyester. WebbDr. Guy Lippens, CNRS Research Director and co-author of the article, adds: “Nuclear Magnetic Resonance (NMR) is an extraordinary biophysical technique for visualizing an enzyme directly in solution. Our study is the first to use NMR as a complementary technique to crystallography and molecular modeling to observe a PETase. office depot girl desk accessories
